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Pradnye

Ayush Tripathi

Chromatography Is A Widely Used Analytical Technique For Separation, Isolation And Identification Of Organic, Inorganic And Biochemical Compounds From Mixtures. The Separated Species Appears As Coloured Bands Or Colourless Spots Which Can Be Developed Into Coloured Spots. This Process Takes Advantage Of The Differing Solvent-solute Interactions. This Is Based On The Principle Of Differential Distribution Of The Components Of A Mixture Between Two Phases, A Stationary Phase And A Mobile Phase. The Stationary Phase May Be Either A Solid Or Liquid And The Mobile Phase May Be A Liquid Or Gas. Paper Chromatography Is A Form Of Partition Chromatography. In Partition Chromatography, The Stationary Phase Is A Thin Liquid Film Adsorbed On The Surface Of An Inert Support And The Mobile Phase Is Either A Liquid Or A Gas. Paper Chromatography Is A Popular Technique For The Separation Of Metal Cations. Paper Is Primarily Cellulose. Here Partition Involves The Distribution Of A Solute Between A Mobile Liquid Phase And Water Molecules Present In The Pores Of The Filter Paper Act As The Stationary Phase And The Mobile Phase Is The Developing Solvent Like Hexane, Toluene, Acetone, HCl , Water Or A Mixture Of Solvents Such As Methanol-acetic Acid Etc. In Which The Paper Is Dipped Marginally. The Ions Are Attracted To The Cellulose In Varying Degrees And The Separation Is Achieved In An Ascending Technique By Upward Movement Of The Solvent By Capillary Action. As The Mobile Phase Moves Through The Spot Containing The Mixture Of Ions Which Has Been Adsorbed, It Dissolves The Components Depending Upon The Solubility And Carries Them Along With It While Moving On The Support. For Separating And Identifying A Mixture Of Metal Ions Whatman No.1 Chromatography Paper Is Used Where A Mixture Of Solvents Like Water, Hydrochloric Acid, Ethyl Acetate And Acetone Is Used As The Mobile Phase Known As The Developing Solvent. The More Soluble Component Of The Mixture In Organic Solvent Distributes More In This Solvent Relative To The Aqueous Phase And Shows Higher Retention Factor (Rf) While The Component Having Lower Solubility Shows Lower Rf Value. Thus The Retention Factor Is Characteristic Of A Particular Species And Is Different In Different Solvents. Rf Values Can Be Calculated Using The Following Expression

Gurvendra Singh

Kyrille Jamias

Manisha Nagesh

Jumah

Cassius Malfoy

Abhaysimha

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Rajpalsinh Bhati Name Signature

Write or Draw digital Signature (Make your own eSign)

F.A.Q About signature

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